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The <t>H2AE92K</t> nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.
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The <t>H2AE92K</t> nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.
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The <t>H2AE92K</t> nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.
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The <t>H2AE92K</t> nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.
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The <t>H2AE92K</t> nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.
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The <t>H2AE92K</t> nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.
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The H2AE92K nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.

Journal: The Journal of Biological Chemistry

Article Title: Mechanisms of DNA methyltransferase 3A1-mediated DNA methylation of nucleosomes

doi: 10.1016/j.jbc.2026.111303

Figure Lengend Snippet: The H2AE92K nucleosome acidic patch mutant inhibits linker DNA methylation and binding by DNMT3A1 . A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes. Data represents the mean and standard deviation of two independent reactions. B , AlphaLISA assays of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers show DNMT3A1 has a lower EC 50 rel on WT nucleosomes compared to H2AE92K nucleosomes.

Article Snippet: A , Michaelis–Menten curves of DNMT3A1 on Epicypher WT and H2AE92K nucleosomes with two 26 bp linkers using 50 nM DNMT3A1 and varied concentrations of nucleosomes.

Techniques: Mutagenesis, DNA Methylation Assay, Binding Assay, Standard Deviation